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In budding yeast, the Rev1 catalytic activity has been shown to be required in vivo for TLS across N 2 -dG-derived lesions such as 4-nitroquinoline-1-oxide (4-NQO) 37 , as well as 1,N 6 -ethenoadenine adducts 38 , but in human cells the scaffolding function of Rev1 appears to play an important role 63, 64

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Notably, an increased level of the PLP protein was detected in the culture treated with 0.01 ng/ml HeS (Fig